Einleitung in Groben Zügen fertig; Ergänzungen an Material/Methoden
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\end{tabularx}
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%\end{center}
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\subsection{Kulturmedien}
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\begin{description}
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\item[2YT] \hfill \\
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\SI{1,6}{\percent} (w/v) Pepton\\
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\SI{1}{\percent} (w/v) Hefeextrakt\\
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\SI{0,5}{\percent} (w/v) Natriumchlorid
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\end{description}
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\subsection{Puffer und Lösungen für den Zellaufschluss}
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\begin{description}
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\item[Aufschlusspuffer] \hfill \\
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\subsection{Puffer und Lösungen für den Westernblot}
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\label{sec:puffer_westernblot}
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\begin{description}
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\item[Transferpuffer] \hfill \\
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\item[TBS] \hfill \\
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\item[TBT] \hfill \\
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\item[Caseinlösung] \hfill \\
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\item[Transferpuffer]\hfill \\
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\SI{25}{\milli\Molar} Tris\\
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\SI{190}{\milli\Molar} Glycin\\
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\SI{10}{\percent} (v/v) Ethanol
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\item[TBS pH 7,5 - 8,0] \hfill \\
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\SI{10}{\milli\Molar} Tris/\ce{HCl}\\
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\SI{150}{\milli\Molar} Natriumchlorid
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\item[TBT pH 7,5 - 8,0] \hfill \\
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\SI{20}{\milli\Molar} Tris\\
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\SI{500}{\milli\Molar} Natriumchlorid\\
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\SI{0,05}{\percent} (v/v) Polysorbat 20
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\item[Caseinlösung pH 7,5 - 8,0] \hfill \\
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\SI{1}{\percent} (w/v) Casein in TBS\\
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\SI{2}{\milli\liter} \SI{2}{\Molar} \ce{NaOH} je \SI{500}{\milli\liter}
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\end{description}
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\subsection{Puffer und Lösungen für den Nachweis der Häminbindung}
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\label{sec:puffer_haeminbindung}
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\begin{description}
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\item[Häminlösung] \hfill \\
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\item[Häminlösung (\SI{1,1}{\milli\Molar})] \hfill \\
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\SI{1}{\milli\gram} Hämin\\
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\SI{50}{\micro\liter} \SI{1}{\Molar} \ce{NaOH}\\
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ad \SI{1,39}{\milli\liter} \ce{dH2O}
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\item[Puffer NP] \hfill \\
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\SI{500}{\milli\Molar} Natriumchlorid\\
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\SI{10}{\milli\Molar} Natriumphosphat pH 7,5
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\item[Waschpuffer pH 6,5] \hfill \\
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\SI{30}{\milli\Molar} BisTris/\ce{HCl} pH 6,5\\
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\SI{200}{\milli\Molar} Natriumchlorid\\
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\SI{0,002}{\percent} (w/v) \ac{PMSF}\\
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\SI{0,003}{\percent} (w/v) Fos-Cholin$^\text{\textregistered}$ 16
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\item[Waschpuffer pH 8,5] \hfill \\
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\SI{30}{\milli\Molar} Tris/\ce{HCl} pH 8,5\\
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\SI{200}{\milli\Molar} Natriumchlorid\\
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\SI{0,002}{\percent} (w/v) \ac{PMSF}\\
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\SI{0,003}{\percent} (w/v) Fos-Cholin$^\text{\textregistered}$ 16
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\item[CD-Puffer] \hfill \\
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\SI{50}{\milli\Molar} Kaliumphosphat pH 7,4\\
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\SI{0,003}{\percent} (w/v) Fos-Cholin$^\text{\textregistered}$ 16
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\end{description}
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\end{singlespace}
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